Function and synthesis of small nucleolar RNAs. cautiously isolated to avoid cytoplasmic contamination. Intranuclear parts were isolated from nuclei by further dissection to separate nucleoli from your chromosomes and interchromatin. Protein Extraction and Western Blotting Nuclear and cytoplasmic components of tissue tradition cells were prepared essentially as explained by Wurtz (1996) . Nuclear draw out … Continue reading Function and synthesis of small nucleolar RNAs